For the past two days we have been working on cells, mitosis, and onion roots! My last post was on a web quest of cells in the stages of mitosis. If you don't know the stages they are:
Interphase: The cell cycle stage between nuclear divisions, when chromosomes are extended and functionally active. The metabolically active non-dividing stage of the cell cycle.
Prophase: The early stage of nuclear division during which chromosomes condense and become visible. The initial stage of mitosis or meiosis in which chromosomes become visible and the spindle apparatus forms.
Metaphase:The stage of mitosis or meiosis in which spindle fibers are attached to kinetochores and the chromosomes are positioned in the equatorial plane of the cell. An intermediate stage of nuclear division when chromosomes align along the equatorial plane of the cell spindle.
Anaphase: The stage of meiosis and mitosis in which sister chromatids (meiosisII) or homologous chromosomes (mitosis and meios
Telophase: The terminal stage of mitosis or meiosis in which chromosomes uncoil; the spindle breaks down; and cytokinesis usually occurs. The late stage of nucleirdivision when daughter
If you want to know more or find more definitions the site below is where I obtained my information.
This post is about the Onion root lab that my group and I worked on!
We had to take onion root tips and place them under a microscope. We needed to find and count the ones in Interphase. Then find and count the ones in Prophase and so on. Then once we counted all the cells we added them up and it gave us our total cells we counted. Once we did that we had to divide the number of cells by the total to get the percentage.
Below is a table we created to help us keep track of our data:
Allium Onion Root Tip | Interphase | Prophase | Metaphase | Anaphase | Telophase | Total |
Number of Cells | 39 | 21 | 9 | 8 | 6 | 83 |
% of Cells | 47% | 25% | 11% | 10% | 7% | 100% |
For our first run we use and Allium Onion root tip. We had 39 cell in interphase, 21 cells in prophase, 9 in metaphase, 8 in anaphase and 6 in telophase. Our total added up to 83 cells. We then divided the number of cells by the total and got the percentages. While my group and I were calculating the percentages, we noticed that the number of cells seemed to decrease as the stages went along, same with percentages. Anyway our next run was on a different cell. Below is our next run.
Mitosis in Onion Root Tip (Is Quadrple Stain) | ||||||
Interphase | Prophase | Metaphase | Anaphase | Telophase | Total | |
Number of Cells | 63 | 34 | 25 | 15 | 13 | 150 |
% of Cells | 42% | 23% | 17% | 10% | 8% | 100% |
For this table we worked with Mitosis in Onion root tips. The Quadrple Stain. We did the same to this table as the first table. Except we had a different total. Our total was 150 cells. In this cell we counted a lot more than the other one, so our total and percentages were a lot higher too. We found that no matter what cell during the stages of mitosis the number of cells in each stage will always decrease, hmm...interesting.
After our group was done collecting data, it was time to make graphs!
Our graphs are on the percentages of the cells in each stage. Our first graph is from our first table.
This was our first graph. Like I said before you can see the percentage decrease with each stage.
If you can't read the labels on the side heres what it says:
Interphase
Prophase
Metaphase
Anaphase
Telophase
Interphase
Prophase
Metaphase
Anaphase
Telophase
Most of the cells stay in interphase and prophase for awhile. There are about (guesstimating here) 12 to 5 cells in metaphase and anaphase. About 5 to 3 cells are in telophase. Our next pie chart show the data from our second table
This is the pie chart for the Quadrple Stain data. The labels on the side say the same as the ones on the first pie chart. The colors are the same to. The only thing that is different is the percentages. this one had more cells than the last pie chart, but the stages still decrease in cells as they move along.
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